Journal:

Article Title: SUSTAINED HYPOXIA MODULATES MITOCHONDRIAL DNA CONTENT IN THE NEONATAL RAT BRAIN

doi: 10.1016/j.freeradbiomed.2007.11.005

Figure Lengend Snippet: A) RT-PCR reaction products generated with RNA isolated from normoxic and hypoxic P14 brains (n=4) are shown for the nuclear PGC-1α and NRF-1 and the mitochondrial transcription factor A Tfam. Bar graphs represent relative signal intensities captured and analyzed by BioRad ChemiDoc XRS Image station with Quantity One software obtained for 3 PCR amplification for each reverse transcription reaction and expressed as mean±SD. * denotes different from normoxic P14, P<0.05. B) Corresponding Western blotting analyses of NRF-1 and Tfam in protein extracts prepared from normoxic and hypoxic P14 brains (n=4). Molecular weight range is indicated for reference and β-actin signal is shown as loading control.

Article Snippet: PCR products were resolved in 1% agarose gels and analyzed by BioRad ChemiDoc XRS Image station with Quantity One software. table ft1 table-wrap mode="anchored" t5 caption a7 Target Gene Accession No. Primer Sequence Product Length NRF1 XM_231566 5′ATGGAGGAACACGGAGTGAC3′ 5′CCACAGAGACTGGAATCCCA3′ 592 bp Tfam NM_031326 5′GGAATGTGGGGCGTGCTAAGAA3′ 5′GCATTCAGTGGGCAGAAGTCCA3′ 869 bp PGC-1α NM_031347 5′TGTGAATGACCTGGACACAGACAGCT3′ 5′CGTCTTTGTGGCTTTTGCTGTTGAC3′ 489 bp β-Actin MN_031144 5′TGCTCCTCCTGAGCGCAAGTACTCT3′ 5′AGAACTTTGGGGGATGTTTGCTCCA3′ 385 bp Open in a separate window RT-PCR primers

Techniques: Reverse Transcription Polymerase Chain Reaction, Generated, Isolation, Software, Amplification, Western Blot, Molecular Weight